Rapid Hygienic Evaluation Of Environmental Genotoxic Carcinogens

Olga OSTASH, State Institution "O.M. Marzeyev Institute for Hygiene and Medical Ecology, National Academy of Medical Sciences of Ukraine", Ukraine
CHERNICHENKO I. 1 , BALENKO N. 1 , VINARSKA E. 2 , GRIGORENKO L. 2

1 Laboratory for carcinogens research, State Institution "O.M. Marzeyev Institute for Hygiene and Medical Ecology, National Academy of Medical Sciences of Ukraine", Kyiv, Ukraine
2 Laboratory for allergic factors research, State Institution "O.M. Marzeyev Institute for Hygiene and Medical Ecology, National Academy of Medical Sciences of Ukraine", kyiv, Ukraine

Purpose: The aim of the work was to determine the principal possibility early immunological, pathomorphological and genotoxic changes in the organism for assessment the chemicals carcinogenicity.
Methods: The experiment was performed on white random-bred male mice. It included 2 series of the investigations: application of benz(a)pyrene on skin in doses (10.5µg; 2.1µg; 0.21µg); peroral administration of benz(a)pyrene and phenol (single dose - 0.1 mg). Genotoxic changes in skin and forestomach were assessed with the help of micronuclear test.
Results: Regardless of routes of administration carcinogen we determined an increase frequency of micronuclei cells and suppression of T-link of immune system during the first month. In the period between the 1t and the 3d months we observed a stabilization of the number of micronuclei cells and deepening of immunosuppression at the expense of the suppression of humoral chain of the immunity.  Repeat increasing of genotoxic effects were found also in later periods of experiment, which is not dependent on the dose of carcinogen, but was associated with induced morphological manifestations of different carcinogenesis stages in target organs.
         Conclusion: Revealed differences in the effect of carcinogen and toxic substances, parallel development and unidirection relative to carcinogenesis of the changes of the indices of mutagenic effect and immunologic reactions and also a presence of the reliable correlative link between them in  early period (during 1 month) only under exposure of benz(a)pyrene doses which induced the tumors of skin and forestomach is an evidence of a possibility of the use of the complex of these indices as an early criteria of the carcinogenicity of genotoxic chemicasl.
         Funding source: The obtained data became a basis for the development of methodic scheme of accelerated testing of the chemical substances for assessment carcinogenicity and hygienic setting of genotoxic carcinogens.