Disregulation Of MicroRNA Expression In Triple Negative Breast Cancer

Izabela ZAWLIK, University of Rzeszow, Poland

1 Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Rzeszow, Poland
2 Laboratory of Molecular Biology, Center for Innovative Research in Medical and Natural Sciences, University of Rzeszow, Rzeszow, Poland
3 Department of Pediatrics, Oncology, Hematology and Diabetology, Medical University of Lodz, Lodz, Poland
4 Department of Head and Neck Cancer Surgery, Medical University of Lodz, Lodz, Poland
5 Department of Surgical Oncology, Cancer Center, Copernicus Memorial Hospital, Lodz, Poland

PURPOSE: Triple-negative breast cancer (TNBC) represent the most common malignancy in women with aggressive behavior pattern, limited treatment options and poor prognosis. Difficulties in therapy TNBC result from a lack of well-characterized molecular profile. There is increasing evidence that epigenetic factors such as microRNAs (miRNAs) are significantly engaged in the development of TNBC.  Expression of microRNAs can serve as a potential biomarkers important for diagnostic, prediction and prognostic in TNBC. The aim of our study was to examine expression of selected miRNAs in TNBC and to assess the relationship between miRNA expression and clinical features.
METHODS: Expression level of 19 selected microRNAs were analyzed by Real Time PCR in   breast tissues of 11 TNBC patients. We evaluated the relations between the miRNAs expression level and lymph node status and age.
RESULTS: We found that in TNBC tissues expression of miR-190a, miR-136-5p and miR-126-5p was significantly reduced, whereas miR-135b-5p and miR-182-5p was significantly increased compared with normal breast tissues. However, there was no association between the miRNAs expression level and lymph node status and age.
CONCLUSIONS: miRNAs expression is deregulated in TNBC. However, clinical implication of miRNAs expression need to be elucidate on a larger population.
FUNDING SOURCE: This study was supported by the grant of the National Center of Science, Poland, 2011/01/B/NZ4/03345