Assessment Of Polymorphisms Of Tumor Suppressor Gene Lysyl Oxidase Among Nepalese Patients Of Oral Submucous Fibrosis

Jyotsna RIMAL, BP Koirala Institute of Health Sciences, Nepal
BHATTARAI N. 2 , SHRESTHA A. 3 , RIJAL S. 4 , KHANAL B. 2

1 Department of Oral Medicine and Radiology, BP Koirala Institute of Health Sciences, Dharan, Nepal
2 Department of Microbiology, BP Koirala Institute of Health Sciences, Dharan, Nepal
3 Department of Public Health Dentistry, BP Koirala Institute of Health Sciences, Dharan, Nepal
4 Department of Internal Medicine, BP Koirala Institute of Health Sciences, Dharan, Nepal

Background: Oral submucous fibrosis (OSF) is a chronic, insidious disease associated with significant functional morbidity and an increased risk for malignancy. The common hypothesis for OSF pathogenesis is the increase of collagen synthesis or reduced collagen degradation in oral sub-epithelial tissues because of chemical, physical/inflammatory irritation. Although available epidemiological evidence indicates that chewing of areca-nut is an important risk for developing of OSF, not all chewers develop OSF. However, no genetic study has been done till date on OSF in Nepalese population.
 
Objective: To explore genetic variability of tumor suppressor genes among the OSF patients and to find relationship between OSF and tumour suppressor gene-lysyl oxidase’s polymorphism among patients attending BPKIHS
 
Methods: A total of 46 samples were collected from 26 patients with OSF and 20 age-sex matched patients undergoing extraction of third molar served as control. A detailed medical, dental history and oral examinations was recorded. Punch biopsy was taken using 6mm punch then was sent to molecular biology laboratory for genetic analysis. 
 
Results: Only 39 samples were suitable for analysis by PCR-RFLP. The mean age was 30.9±12.09. Out of 39 samples, 27 were OSF cases and 12 were control. The fingerprinting analysis revealed three different genotypes ie homozygous G⁄G allele, A⁄A allele and heterozygous G⁄A alleles showed one, two and three bands respectively after pstI digestion. Further analysis showed that heterozygous allele G/A was predominantly found in case and control whereas genotype A/A and G/A were found in control and case respectively. Analysis of demographic information documented that habit of smoking/chewing tobacco was predominantly found in genotype G/G as compared to others.
 
Conclusion: This study explored possible genetic marker of OSF in Nepalese population. However, further studies with large number of samples are required to validate these developed simple molecular tools which are easy to perform and analyse.